| Johnson City KinderCare | 906 Sunset Dr, Johnson City, TN 37604 | Preschool |
| 21st Century Homework Club | 907 Kingsprings Road, Johnson City, TN 37601 | Center |
| Andrew Johnson Eagle Watch | 1001 Ormond Drive, Kingsport, TN 37664 | Center |
| Apple Tree Play House Learning Center | 252 Westfield Dr., Kingsport, TN 37664 | Center |
| Ashley Academy | 1502 Knob Creek Road, Johnson City, TN 37604 | Center |
| Babyzone Child Care Center | 103 L.p. Auer Rd., Johnson City, TN 37604 | Center |
| Boone Trail Early Learning Center | 1985 Carroll Creek Rd., Johnson City, TN 37615 | Preschool |
| Boones Creek Child Development Center | 1806 Old Gray Station Rd, Johnson City, TN 37615 | Center |
| Boones Creek Elementary - SACC | 348 Boones Creek, Johnson City, TN 37615 | Center |
| Boones Creek Head Start | 348 Christian Church Rd, Johnson City, TN 37615 | Preschool |
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| Carver Head Start | 500 Washington St, Johnson City, TN 37601 | Center |
| Central Baptist Preschool | 300 North Roan Street, Johnson City, TN 37601 | Preschool |
| Cherokee Christian Learning Center | 2615 South Roan Street, Johnson City, TN 37601 | Preschool |
| Children First Head Start Center | 837 Pardee St., Johnson City, TN 37601 | Preschool |
| Coalition For Kid's - Kid's In Action | 2308 Watauga Rd., Johnson City, TN 37601 | Center |
| Coalition For Kids Mountain View Elementary | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Coalition For Kids North Side Elementary | 1000 N. Roan St., Johnson City, TN 37601 | Center |
| Colonial Heights Baptist Child Care | 108 Colonial Heights Road, Kingsport, TN 37663 | Preschool |
| Colonial Heights Presbyterian Day School | 213 Colonial Heights Road, Kingsport, TN 37663 | Center |
| Colonial Heights United Methodist Preschool | 631 Lebanon Road, Kingsport, TN 37663 | Preschool |
| Early Childhood Learning Center - Palmer | 1609 Ft. Henry Drive, Kingsport, TN 37664 | Center |
| Early Learning Center - First United Methodist Church | 900 Spring Street, Johnson City, TN 37604 | Center |
| Encore Senior Child Care Center | 1800 Legion Dr., Kingsport, TN 37664 | Center |
| Etsu Child Study Center | 2101 Signal Drive, Johnson City, TN 37604 | Center |
| Fairmont Elementary - Educare | 1405 Lester Harris Road, Johnson City, TN 37601 | Center |
| First Christian Church Preschool | 200 Mountcastle Drive, Johnson City, TN 37601 | Preschool |
| First Presbyterian Church Preschool | 105 South Boone Street, Johnson City, TN 37604 | Preschool |
| Gray Elementary - SACC | 755 Gray Station Road, Johnson City, TN 37615 | Center |
| Gray Elementary Pre-kindergarten | 755 Gray Station Road, Johnson City, TN 37615 | Center |
| Gray United Methodist Church Preschool | 2108 Oak Street, Johnson City, TN 37615 | Preschool |
| Harmony Head Start Center | 1019 Painter Road, Jonesborough, TN 37659 | Preschool |
| Holy Mountain Daycare | 3121 Ashley Street, Kingsport, TN 37664 | Center |
| Hospitots Child Care Center | 1719 West Market Street, Johnson City, TN 37604 | Center |
| Indian Trail Intermediate School Educare | 307 Car-mol Drive, Johnson City, TN 37601 | Center |
| Jack And Jill | 1620 Old Boones Creek Rd, Johnson City, TN 37615 | Center |
| John Adams Elementary Early Child Learning & SACC | 2727 Edinburgh Channel Road, Kingsport, TN 37664 | Center |
| Johnson City Kidz Clubhouse | 408 West Market St., Johnson City, TN 37604 | Center |
| Johnson City Prek At Fairmont Elementary | 1405 Lester Harris Road, Johnson City, TN 37601 | Preschool |
| Jonesborough Elementary - SACC | 306 Forest Drive, Jonesborough, TN 37659 | Center |
| Jonesborough Middle School SACC | 308 Forest Drive, Jonesborough, TN 37659 | Center |
| Kiddie Kollege | 2308 Sherwood Road, Kingsport, TN 37664 | Center |
| Kidz Academy | 5452 Highway 11e, Piney Flats, TN 37686 | Center |
| Kingsport City Pilot Pre-kindergarten Program | 1690 Fort Henry Drive, Kingsport, TN 37664 | Preschool |
| Lake Ridge Elementary - Educare | 1001 Lake Ridge Square, Johnson City, TN 37601 | Center |
| Lake Ridge Elementary - Preschool | 1001 Lake Ridge Square, Johnson City, TN 37601 | Preschool |
| Lake Terrace Head Start | 2450 Mint Hill Rd, Johnson City, TN 37601 | Preschool |
| Lamar Elementary - SACC | 3261 Highway 81s, Jonesborough, TN 37659 | Center |
| Lamar Elementary Pre-kindergarten | 3261 Highway 81 South, Jonesborough, TN 37659 | Preschool |
| Leaps Jump In - Jump Ahead | 820 West Market Street, Johnson City, TN 37604 | Center |
| Learn And Grow Childcare Center | 604 Boones Creek Road, Jonesborough, TN 37659 | Center |
| Lincoln Preschool | 1000 Summer Street, Kingsport, TN 37664 | Preschool |
| Little Bo Peep Learning Center | 305 West Main, Jonesborough, TN 37659 | Center |
| Little Buccaneers Student Child Care Center | 807 University Parkway, Johnson City, TN 37614 | Center |
| Little Tigers Preschool & Enrichment | 305 Forest Dr, Jonesborough, TN 37659 | Preschool |
| Midway Preschool And Day Care | 102 Post Oak Drive, Kingsport, TN 37663 | Preschool |
| Mother Goose Day Care Center | 709 N. Boone St., Johnson City, TN 37604 | Center |
| Mountain View Baptist Daycare & Learning | 4005 Bristol Hwy., Johnson City, TN 37602 | Center |
| Mountain View Elementary Educare | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Mountain View United Methodist Day Schol | 4405 Orebank Road, Kingsport, TN 37664 | Center |
| Mrs. Belinda's Learning Center | 514 Kendricks Creek Rd, Kingsport, TN 37663 | Center |
| Munsey Memorial Preschool | 201 E. Market St., Johnson City, TN 37601 | Preschool |
| North Side Elementary School Educare | 1000 North Roan Street, Johnson City, TN 37601 | Center |
| Northside Elementary Pre-kindergarten | 1000 N. Roan, Johnson City, TN 37601 | Preschool |
| Palmer Center Preschool Program | 1609 Ft. Henry Drive, Kingsport, TN 37664 | Preschool |
| Parkway Preschool | 116 Claude Simmons Road, Johnson City, TN 37604 | Preschool |
| Persimmon Ridge Head Start | 1521 Persimmon Ridge Rd, Jonesborough, TN 37659 | Preschool |
| Pinecrest Head Start | 500 Angel Drive, Johnson City, TN 37601 | Preschool |
| Playstation - The Kids Depot | 1006 South Broadway, Johnson City, TN 37601 | Center |
| Princeton Preporatory | 504-a Princeton Road, Johnson City, TN 37601 | Center |
| Regency Child Care | 132 Leslie Court, Kingsport, TN 37663 | Center |
| Ridgeview Elementary Idea 619 Pre-kindergarten | 252 Sam Jenkins Road, Johnson City, TN 37615 | Preschool |
| Ridgeview School Age Child Care | 252 Sam Jenkins Road, Johnson City, TN 37615 | Center |
| Rise Up! | 1500 East Millard Street, Johnson City, TN 37601 | Center |
| Rock Springs Elementary School Pre-kindergarten | 1238 Moreland Drive, Kingsport, TN 37664 | Preschool |
| Science Hill Preschool | Technology Center, Johnson City, TN 37601 | Preschool |
| Shepherd's Pasture Preschool | 1112 Cherokee Road, Johnson City, TN 37604 | Preschool |
| Shining Stars Christian Learning | 2615 South Roan Street, Johnson City, TN 37604 | Center |
| Small Steps Children's Academy | 1201 East Chilhowie Ave., Johnson City, TN 37601 | Center |
| Sonshine Child Development Center | 171 Promise Land Drive, Johnson City, TN 37615 | Preschool |
| St. Mary's School | 2211 East Lakeview Drive, Johnson City, TN 37601 | Center |
| Stepping Stones Day Care And Preschool | 216 East Market St., Johnson City, TN 37601 | Preschool |
| Sulphur Springs Community Child Care | 1432 Gray-sulphur Springs Rd., Jonesborough, TN 37659 | Center |
| Sulphur Springs School - SACC | 1518 Gray Station/sulphur Springs Rd., Jonesborough, TN 37659 | Center |
| Sundale Preschool | 2106 Sundale Road, Johnson City, TN 37604 | Preschool |
| Tiny Town | 1012 Knob Creek Road, Johnson City, TN 37604 | Center |
| Tri Cities Christian Preschool And After | 1500 Highway 75, Blountville, TN 37617 | Preschool |
| Tri-cities Christian Preschool & Aftercare - Gray | 2102 Maple Dr, Johnson City, TN 37615 | Preschool |
| Waverly Road Child Care Center | 1415 Waverly Rd, Kingsport, TN 37664 | Center |
| Wesley Memorial United Methodist Church Preschool | 225 Princeton Road, Johnson City, TN 37601 | Preschool |
| YMCA - Holston Elementary School | 2348 Highway 75, Blountville, TN 37617 | Center |
| YMCA - Miller Perry School Age | 904 Fordtown Road, Kingsport, TN 37663 | Center |
| YMCA - Rock Springs Elementary School | 1238 Moreland Drive, Kingsport, TN 37663 | Center |
| Yaya-Raha Learning Center | 3828 Alderwood Drive, Kingsport, TN 37664 | Center |